Bioparticles Encapsulation Efficiency Analysis


Encapsulation efficiency is one of the important parameters to evaluate the success of drug delivery system. As an undisputed global leader in the field of bioparticles developing and manufacture, CD Bioparticles can offer reliable analysis service to quantify the encapsulation efficiency for different drug carriers.

Encapsulation Efficiency Introduction

The encapsulation efficiency (EE%) is the percentage of drug that is successfully entrapped into drug carriers. It is defined by the concentration of the incorporated material (such as active ingredients, drugs, fragrances, proteins, pesticides, antimicrobial agents, etc.) detected in the formulation over the initial concentration used to make the formulation. Therefore, the encapsulation efficiency (%) is calculated by the following formula:

Encapsulation efficiency (%) = [1-(unencapsulated drug/total drug)] ×100

The encapsulation efficiency can be influenced by (i) the partition coefficient of the target molecule in the solvents used in the preparation of the formulation, (ii) the method used to carry out the encapsulation process (temperature, pH, mechanical stress), and (iii) the size distribution of the capsules.

Figure 1. Liposome encapsulation efficiency measurement. (Eiichi Yamamoto, etc. International Journal of Pharmaceutics (2018) Volume 536, Issue 1, Pages 21-28)

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CD Bioparticles provides reliable service for drug separation and encapsulation efficiency analysis. To start a determination experiment, the first step is the separation between the encapsulated drug (within the carrier) and the free drug. Methods such as centrifugation, exclusion column, and dialysis membranes have been used widely for separation. Techniques used for drug quantification depend on the nature of the encapsulant and include spectrophotometry, fluorescence spectroscopy, enzyme-based methods, and electrochemical techniques. Other methods such as HPLC or FFF can also be applied for the determination of the encapsulation efficiency. Please contact us for a free consultation to design and customize analytical method suitable for your drug molecules.

CD Bioparticles offers a full set of service for bioparticles encapsulation efficiency analysis. For more detailed information, please feel free to contact us or directly send us an inquiry.

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References:
1. Huang Y Z, Gao J Q, Liang W Q, et al. Preparation and characterization of liposomes encapsulating chitosan nanoparticles. Biological and Pharmaceutical Bulletin (2005) 28(2): 387-390.
2. Jyothi N V N, Prasanna P M, Sakarkar S N, et al. Microencapsulation techniques, factors influencing encapsulation efficiency. Journal of microencapsulation, (2010) 27(3): 187-197.
3. Sun B, Chiu D T. Determination of the encapsulation efficiency of individual vesicles using single-vesicle photolysis and confocal single-molecule detection. Analytical Chemistry (2005) 77(9): 2770-2776.

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