Fluorescent Lipids

Fluorescent lipids, which are mainly used for tracking and imaging, have been widely used in the observation of viral membrane fusion, providing a sensitive method to study fusion mechanisms. Due to the wealth of data on liposome fusion, many fusion detection methods have been devised, including fluorescence probe redistribution, fluorescence quenching, fluorescence resonance energy transfer, and photo-sensitive tagging.  The interplay between the viral fusion mechanism and the membrane can be observed by photo-sensitive tagging of interacting fragments of the envelope protein with hydrophobic probes. Fluorescent lipids thus provide a wide range of fusion analyses and powerful tools to generate accurate quantitative data in real-time to elucidate the complex process of viral fusion.

CD Bioparticles' services with customized delivery strategies, precise designs and modifications of drugs or drug-contained cargos, and advanced technical platforms can help you to solve:

The challenges you might meet:

• Limited options for tracking and detection of fusion, pore forming, and cell uptake (e. g. macrophage) experiments
• Hard to track and image the distribution of the lipids
• Limited wavelength coverage for your tracking and detection experiments Issue of quenching or fluorescence compensation
• Non-active fluorescent lipids which cannot be conjugated with other molecules
• Tedious chemical synthesis, formulation, and purifications

Key features:

• Fluorescent Sterols
• Fluorescent PEG Lipids
• Fluorescent Phospholipids
• Fluorescent Glycerolipids
• Fluorescent Sphingolipids
• Other

Key benefits:

• Wide coverage of fluorescent lipophilic tracer for tracking and detection of the liposomes
• Good biocompatibility and low immunogenicity
• Different net charges of the fluorophores for parallel studies
• Multi-functional-group lipid useful for further conjugation and crosslinking
• Drug-loaded or non-loading fluorescent liposomes for positive control and negative control of your drug delivery tests
• Available post-insertion of the PEGylation to improve the circulation of the liposomes
• Precise control of the fluorophore substitution, well-control of the intensity-concentration dependence
• Suitable for in vitro and in vivo experiments
• Ready-to-use

Application candidates:

• As a virus membrane fusion tracer probe
• Experiments involving pore formation and membrane destruction investigate pore formation or destruction by external factors such as detergents, peptides, etc.
• Tracking and detecting cellular uptake processes such as endocytosis
• Conjugation with biomolecules for tracking and detecting the biomolecules PK/PD analysis