Diazo Biotin Cleavable Linkers

Diazo Biotin Cleavable Linkers are biotinizing reagents that contain an extended PEG spacer group and a terminal primary amine group. Azides can be chemically coupled to propyl, DBCO, BCN, and other labeled molecules by click-binding to form stable triazole bonds. Extended PEG spacers increase the water solubility of the printed contents and help reduce steric hindrance in binding to streptavidin and other proteins. After the alkylating protein is trapped on the Streptavidin beads, only the drug-binding protein is released. The mild elution conditions ensure that any endogenous biotin or other non-specific cell material remains on the Streptavidin beads. Diazo Biotin Cleavable Linkers have been used in chemical proteomics studies.

CD Bioparticles' services with customized delivery strategies, precise designs and modifications of drugs or drug-contained cargos, and advanced technical platforms can help you to solve:

The challenges you might meet:

• Drugs have a shorter circulation time in the blood
• Drugs cannot penetrate deep into tumor tissue to reach tumor cells
• Linkers are unable to lyse rapidly in target tumor tissue causing toxic accumulation
• Instability of linkers causes damage to normal tissue cells
• The immunogenicity of linkers affects the efficacy of target drugs
• Intracellular drug release is difficult to achieve
• Cleavable linkers cannot break under mild conditions

Key features:

• Diazo Biotin-PEG3-alkyne
• Diazo Biotin-PEG3-azide
• Diazo Biotin-PEG3-DBCO

Key benefits:

• Low immunogenicity
• High tumor affinity and specificity
• Increase the hydrophilicity of the loaded drugs
• High bioavailability and biocompatibility
• Mild cracking conditions
• Suitable for in vitro and in vivo experiments
• Ready-to-use

Application candidates:

• Mass spectrometry target recognition
• Protein–protein interactions
• Activity-based protein profiling
• Elucidation of drug targets and their binding sites
• Study on covalent metabolite interactions