Fluorescent Liposomes

CD Bioparticles’ products with deep understanding of the strategies and the applications of various of delivery cargos with precise designs and advanced technical platforms can help you to solve:

The challenges you might meet:

  • Limited options for tracking and detection of fusion, pore forming, and cell uptake (e.g. macrophage) experiments
  • Hard to track and image the distribution of the liposomes
  • Limited wavelength coverage for your tracking and detection experiments
  • Issue of quenching or fluorescence compensation
  • Non-active fluorescent liposomes which cannot be conjugated with other molecules
  • Tedious chemical synthesis, formulation, and purifications

Fluorescent Liposomes Key Features

  • Fluorescent Plain Liposomes
    • Fluorescent control formulations for many different types of liposome formulations
  • Fluorescent Reactive Liposomes
    • Surface reactive fluorescent liposomes for conjugation of active biomolecules, useful for tracking and targeting simultaneously
    • Amine reactive fluorescent liposomes
    • Biotinylated fluorescent liposomes
    • Carboxylic acid reactive fluorescent liposomes
    • DBCO or Azide reactive fluorescent liposomes (Click chemistry)
    • Folate fluorescent liposomes
    • Sulfhydryl reactive fluorescent liposomes
  • Fluorescent Drug Loaded Liposomes
    • Clodronate encapsulated fluorescent liposomes
    • ATP encapsulated fluorescent liposomes, Lyophilized
    • Doxorubicin encapsulated fluorescent/plain liposomes
    • PEGylated fluorescent liposomes
  • Fluorescent Liposomes for DNA/RNA Delivery
    • DOTAP based
    • DDAB based
    • DOTMA based
    • DC-Cholesterol based
    • GL-67 based
  • Fluorescent options:
    • DiA (ex/em: 456/590 nm), DiD (ex/em: 644/665 nm), DiI (ex/em: 549/565 nm), DiO (ex/em 484/501 nm), DiR (ex/em: 750/780 nm), NBD headgroup tagged lipid (ex/em: 460/535 nm), NBD fatty acid tail tagged lipid (ex/em: 460/534), rhodamine headgroup tagged lipid (ex/em: 560/583 nm) , dansyl headgroup tagged lipid (ex/em: 336/513 nm) and pyrene headgroup tagged lipid (ex/em: 351/379 nm)
  • Remote radioactive divalent cations loadable DOTA liposomes

Fluorescent Liposomes Key benefits:

  • Wide coverage of fluorescent lipophilic tracer for tracking and detection of the liposomes
  • Different net charges of the fluorophores for parallel studies
  • Multi-functional-group lipid useful for further conjugation and crosslinking
  • Drug-loaded or non-loading fluorescent liposomes for positive control and negative control of your drug delivery tests
  • Available post-insertion of the PEGylation to improve the circulation of the liposomes
  • Precise control of the fluorophore substitution, well-control of the intensity-concentration dependence
  • Ready-to-use

Fluorescent Liposomes Application candidates:

  • Fusion experiments of two separate membranes.
    • Co-encapsulating NBD/Rhodamine
    • Encapsulating self-quenching octadecyl Rhodamine B
  • Experiments that involve pore formation and disruption of the membrane, to study pore formation or disruption by external factors such as detergents, peptides, etc.
    • Containing various sizes of dextran-FITC dye
  • Tracking and detection of cell uptake (e.g. macrophage)
    • Mannosylated fluorescent controls
    • Standard fluorescent controls: single fluorescence or multi-fluorescence
  • Conjugation with biomolecules for tracking and detecting the biomolecules
  • PK/PD analysis
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