Lipid Nanoparticle Formulation and Development for mRNA Vaccine Delivery


Introduction

Recently, mRNA vaccines have drawn extensive attention due to its potency to tackle various unmet therapeutical issues that are unable to be solved by traditional vaccine technologies. For example, live-attenuated vaccines sometimes exhibit safety drawbacks that could potentially induce pathogenic diseases. While subunit vaccines have been developed as an improved safe alternative, they are less efficient and mostly take effect in the presence of adjuvants.1

Although mRNA vaccines combine the advantages of the abovementioned vaccines and avoid of their risks, they still have to face some major issues including insufficient intracellular delivery efficiency and instability. To this end, several strategies have been developed to bypass such problems, and lipid nanoparticles (LNPs) stand as the most frequently used non-viral vector for the merits of efficiently bind and condense RNA, protect against degradation in the extracellular space and localize the payload at the membrane of the desired target cell, followed by cellular uptake and endosomal escape into the cytosol.2 Table 1 was taken from reference 3 which lists out many of the currently published lipid nanoparticles mRNA vaccine.

Table 1. Overview of published lipid nanoparticles mRNA vaccines used in vivo3

LNPs generally consist of a combination of different lipids which form an aqueous core. Most LNP are designed to carry cationic charge to efficiently combine with the negatively charged mRNA.4 However, several studies have reported that cationic lipids bearing a permanent positive charge are more toxic and less efficient, the potency of LNPs has been advanced significantly with the development of new, ionizable lipids.5 They usually contain amine groups which maintain a neutral or mildly cationic surface charge at physiological pH, thereby reducing nonspecific lipid–protein interactions and facilitating oligonucleotide release in the cytosol. Figure 1 was taken from reference 3 which illustrates the rationale of how LNP mRNA vaccine functions.

Since the most important targets for mRNA vaccines are professional antigen presenting cells (APCs), with dendritic cells (DCs) likely being the most relevant cell type, LNPs can be decorated to target APCs compared to other delivery methods. It has been proved that DCs are studded with different receptors, including lectins that recognize carbohydrate moieties present on many pathogens, and are involved in antigen capture and presentation. A wide variety of different DC receptors have been identified, including the mannose receptor, DC-SIGN, DEC-205 and Langerin. In recent years, these receptors have been characterized and used for targeted protein and protein–LNP vaccines, and could be further represented as LNP mRNA vaccine targets. In fact, mannosylated mRNA–LNPs coding for MART-1 have exhibited decent vaccination rate compared to the non-decorated analogs.6

Figure 1. Antigen presentation on MHC I and II pathways in dendritic cells. (A) Endogenous proteins with pathogen or self origin are primarily displayed on the MHC I pathway. These proteins are degraded into smaller peptides by the proteasome. The peptides are transported into the endoplasmic reticulum for loading onto the MHC class I molecules. This MHC I–peptide complex is then displayed at the cell surface to CD8 T-cells. (B) On the other hand, proteins that enter the cell on the endocytic route are displayed on the MHC II pathway. For this purpose, the MHC class II molecules are protected with the invariant chain (Ii) from binding to endogenous peptides in the endoplasmic reticulum. The MHC II-Ii complex is then exported through the Golgi to the MIIC/CIIV compartment, where the invariant chain is replaced with antigens. The MHC II–peptide complex is then displayed at the cell surface to CD4 T-cells.

Besides, adjuvants were usually required for conventional vaccination to enhance the immune response which eventually led to successful vaccination. LNPs could serve as the adjuvants themselves. LNPs containing cationic lipids, such as 1,2-dioleyl-3-trimethylammonium-propane chloride salt, have been shown to activate Toll-like receptor 4 (TLR4) and induce a strong proinflammatory response with Th1 type cytokines, including IL-2, IFN-γ, and TNF-α.7 However, most of the adjuvants are tested with protein-LNP vaccine.

Future Perspectives of LNP mRNA Vaccines

  • mRNA vaccines are currently evaluated in clinical trials, albeit not formulated in LNPs.
  • LNP delivery of siRNA is already in clinical trials, and lessons from these experiences can be helpful for the translation of mRNA vaccines.
  • mRNA vaccines are currently evaluated for applications in cancer immunotherapy, with prophylactic vaccines applications to follow.

Challenges You Might Have:

  • Traditional vaccine might induce major immunogenicity issues in human body
  • Regular mRNA vaccine shows compromising ability in administration, trafficking, and delivery in clinical trials

Key Services We Can Offer:

  • Design and screen the suitable cationic liposomal cargos for mRNA vaccine encapsulation and delivery
  • Optimize the liposomal cargos for your R&D purposes
    • For imaging and tracking purpose
    • For active targeting purpose
  • Lipid library development for mRNA vaccine development
  • Function and performance analysis and evaluation

Key Features:

  • LNP encapsulated mRNA vaccine can be delivered to the cytosol efficiently
  • DC cells targeting is essential for mRNA-LNP vaccines
  • Particle size is the key parameter that determines the LNP accumulation in DCs
  • LNP can be designed to target the DCs via chemical modification or ligand conjugation
  • Adjuvants can be incorporated in LNPs to assist in immune activation
  • LNP synthesis/formulation (e.g. microfluidic device) is robust and upscalable

Key Benefits

  • Highly stable as the LNP shield protects mRNA from degrading
  • Large payloads and low risks compared to other administration pathways/vectors
  • Ease for future modification to enhance the delivery efficiency

References:
1. Crawford NW, Bines JE, Royle J, Buttery JP. Optimizing immunization in pediatric special risk groups. Expert Rev. Vaccines 10(2), 175–186 (2011).
2. Whitehead KA, Langer R, Anderson DG. Knocking down barriers: advances in siRNA delivery. Nat Rev Drug Discov. 2009 Feb;8(2):129-38. doi: 10.1038/nrd2742. Erratum in: Nat Rev Drug Discov. 2009 Jun;8(6):516. PMID: 19180106; PMCID: PMC7097568.
3. Reichmuth AM, Oberli MA, Jaklenec A, Langer R, Blankschtein D. mRNA vaccine delivery using lipid nanoparticles. Ther Deliv. 2016;7(5):319-34. doi: 10.4155/tde-2016-0006. Erratum in: Ther Deliv. 2016 Jun;7(6):411. PMID: 27075952; PMCID: PMC5439223.
4. Mintzer MA, Simanek EE. Nonviral vectors for gene delivery. Chem Rev. 2009 Feb;109(2):259-302. doi: 10.1021/cr800409e. PMID: 19053809.
5. Kanasty R, Dorkin JR, Vegas A, Anderson D. Delivery materials for siRNA therapeutics. Nat Mater. 2013 Nov;12(11):967-77. doi: 10.1038/nmat3765. PMID: 24150415.
6. Perche F, Benvegnu T, Berchel M et al. Enhancement of dendritic cells transfection in vivo and of vaccination against B16F10 melanoma with mannosylated histidylated lipopolyplexes loaded with tumor antigen messenger RNA. Nanomed. Nanotechnol. Biol. Med. 7(4), 445–453 (2011).
7. Kedmi R, Ben-Arie N, Peer D. The systemic toxicity of positively charged lipid nanoparticles and the role of Toll-like receptor 4 in immune activation. Biomaterials. 2010 Sep;31(26):6867-75. doi: 10.1016/j.biomaterials.2010.05.027. Epub 2010 Jun 11. PMID: 20541799.

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