CD Bioparticles provides custom services for Lipid Nanoparticles (LNPs) formulation design and drug encapsulation. Together with advanced preparation technologies for various liposomes, scientists in CD Bioparticles are proficient in producing LNPs for various types of drug loading, especially for nucleic acid drug (eg siRNA, miRNA, mRNA and plasmid DNA, et al.). We can provide the service of formulation design depending on the application, drug encapsulation, physical and chemical properties, in vitro and in vivo characterization according to customer's project needs.
LNPs is a special cationic liposome, which is composed of ionizable lipids (such as DOTMA, MC3) helper lipids (such as phospholipids), cholesterol, and/or PEGylated lipids, as shown in Figure 1. The use of ionizable lipid resulted in the high encapsulation efficiency of nucleic acids because of the electrostatic interactions. However, the changeable charge depending on the pH of the environment gives the LNPs irreplaceable excellent performance.
Figure 1.Schematic representation of mRNA lipid nanoparticles
The specific features of LNP include:
The applications of LNPs include[5-6]:
LNPs formulation design: we customize LNPs design based on our clients' demand by varying lipid structure (both commercialized and early developed), lipid compositions, vesicle sizes, surface charges, etc.
LNPs encapsulation: we employ customized protocols to encapsulate drug molecules into LNPs by several different methods with high encapsulation efficiency.
LNPs-drug complex analysis: we can offer comprehensive analysis of LNPs before and after encapsulation, which includes visual appearance, size distribution, stability, zeta potential, lamellarity, entrapment efficiency, and release rate.
LNPs-drug biological activity characterization: we can control and analysis the cell uptake efficiency and the cytotoxicity by the lipid structure, charge ratio,etc.