Electron-rich ligands such as histidine, tryptophan, or cysteine show relatively high affinity for electropositive transition metals, including Co2+, Ni2+, Cu2+, and Zn2+. This can be used to improve and control the binding of different histidine-tagged peptides or proteins to liposomes containing metal-chelating lipids. Therefore, immunoliposomes can be generated using nickel-chelating lipids such as Ni-nitrilotriacetic acid (NTA) and 1,2-dioleoyl-sn-glucero-3-[N-(amino-1-carboxypentyl)-iminodiacetic acid) succinyl] (DOGS-NTA aka DGS-NTA), and His-tagged proteins or peptides. Proteins are reversibly anchored to liposomes when liposomes bearing Ni-NTA headgroups are bound to His residues (usually at the N- or C-termini of proteins). CD Bioparticles has developed Ni-responsive liposomes for the conjugation of His-tagged peptides and proteins.
CD Bioparticles manufactures and supplies Ni-responsive liposomes for drug delivery research. Contact us to find out how Ni-reactive liposomes can help your work.
|Product Name||Catalog||Lipid composition||Liposome Size||Price|
|Clipos™ NTA Liposomes, Non PEGylated||CDEIMS-1512||L-a-PC/Cholesterol/1,2-Dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl)iminodiacetic acid)succinyl] (nickel salt) (69/30/1 molar ratio)||100 nm||INQUIRY|