Clipos™ MPL Adjuvant Encapsulated Liposomes

Catalog:CDEPG-15

Unit Size:2 mL, 10 mL

Specifications

Description	Maleimide-reactive, PEGylated, with MPL Adjuvant. Immunogenic PEGylated liposomes containing free maleimide functional group for maleimide-thiol conjugation, plus TLR4 agonist MPL for adjuvant activity. The liposomes are co-formulated with 1 mol% DSPE-mPEG2000-maleimide, and supplied in a pH-optimized buffer for direct conjugation of the thiol-containing molecule of choice. Conjugation can be achieved by incubating the liposomes with the activated thiol-containing compound which has been reduced by a disulfide bond reducing agent such as (tris(2-carboxyethyl)phosphine) (TCEP), which can be purchased as an add-on. The liposomes also contain 1 mol% of the Toll-like receptor 4 (TLR4) agonist monophosphoryl lipid A (MPL), which is effective as an immune-stimulating adjuvant. This product is particularly useful in preclinical applications including vaccination, immunotherapy, or other areas of immunology research, especially in situations where a immunologically-active agent is meant to be administered for the purpose of stimulating an immune response. The surface-reactive maleimide head group enables the conjugation of a wide range of thiol-containing molecules, which could encompass antigens, immunomodulatory drugs (IMiDs), nucleic acids, or other investigational agents.
Lipid composition	HSPC/Chol/DSPE-mPEG2000/DSPE-mPEG2000-maleimide/MPL (49/45/4/1/1 molar ratio)
Liposome Size	70-120 nm
Lipid Molar Concentration	30 mM
Inside Buffer	PBS, pH 6.8
Outside Buffer	PBS, pH 6.8
Storage	2 °C to 8 °C (do not freeze)
Shelf-life	6 months