Clipos™ mCherry mRNA Lipid Nanoparticles

Catalog:CDPM02

Unit Size:200 μL

INQUIRY Datasheet

Specifications
Description mCherry mRNA encodes the fluorescent protein, mCherry, which is derived from DsRed, a protein found in Discosoma sp. mCherry is a monomeric fluorophore with a peak absorption at 587 nm and emission at 610 nm. It is stable and resistant to photobleaching. The mRNA is capped using CleanCap, which results in the naturally occuring Cap 1 structure with high capping efficiency. It is polyadenylated, modified with 5-methoxyuridine and optimized for mammalian systems. It mimics a fully processed mature mRNA. mRNA isolated in sodium citrate, sodium acetate, lipid mix in ethanol, and PBS.
Encoding mRNA mCherry
In Vitro QC (Cell Types) HEK293S
Technology Applications Control
Excitation 587 nm
Emission 610 nm
Storage Store at 4°C.
Technical Notes Work with mRNA-LNP on ice. It is important to minimize the time that the product spends at room temperature; after handling product during experiments, return immediately to ice.
Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container; it is important not to spin for too long as this may rupture mRNA-LNP’s. Do not vortex.
mRNA-LNP products should only be handled with certified RNAase-free reagents and consumables; use of filtered pipette tips is highly recommended.
Usage 1. Prior to transfection, in a 12 well culture plate, plate your cells at [0.5E6 cells/ml] in a total of 1ml per well. Ensure the cells you are using are viable and healthy. Try not to let your cells sit for longer than 5 minutes prior to transfection; cell clumping at the time of transfection may reduce transfection efficiency.
2. Briefly pipette mRNA-LNP mix up and down to resuspend. Add 20ul of the mRNA-LNP mix dropwise directly to your 1ml culture. Gently tilt plate back and forth to mix (not necessary if you are using cells which will be immediately placed back into a shaker) Place your transfected cells back into their original culture conditions.
3. Check cell expression by FACS at 24hr intervals after transfection.
*Note: This is a generalized protocol for transfection using mammalian cells. Transfection volume may be scaled down/up proportionately.
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