Clipos™ ANTI-HER2 mRNA Lipid Nanoparticles

Catalog:CDPM08

Unit Size:200 μL

INQUIRY Datasheet

Specifications
Description This product utilizes the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of the Anti-HER2 mRNA to a variety of mammalian cells, including cell cultures which may typically be prone to difficulties in expressing desired proteins. The lipid nanoparticles used are formulated with, SM-102, DSPC, cholesterol, and DMG-PEG2000 at optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. HER2 is a surface protein involved in normal cellular growth. Over expression of this protein can signal uncontrolled cell growth which can lead to multiple cancers and complications. This makes HER2 protein an ideal target for diagnosis and treatment. This mRNA-LNP transfects the ScFv (single-chain variable fragment) of anti-HER2 linked to a 2nd generation CAR (Chimeric Antigen Receptor) containing CD8 hinge and transmembrane domains, and the 4-1BB and CD3ζ signaling domains. mRNA encapsulated in lipid nanoparticle, primarily in PBS (-ca, -mg). Dilute levels of: sodium acetate, ethanol, free mRNA & free lipids.
Lipid composition SM-102/DSPC/cholesterol/DMG-PEG2000
Encoding mRNA xHER2 CAR
In Vitro QC (Cell Types) T cells, HEK293S
Technology Applications CAR-T
Storage Store at 4°C
Technical Notes Work with mRNA-LNP on ice. It is important to minimize the time that the product spends at room temperature; after handling product during experiments, return immediately to ice.
Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container; it is important not to spin for too long as this may rupture mRNA-LNP’s. Do not vortex.
mRNA-LNP products should only be handled with certified RNAase-free reagents and consumables; use of filtered pipette tips is highly recommended.
Usage 1. Prior to transfection, in a 12 well culture plate, plate your cells at [0.5E6 cells/mL] in a total of 1 mL per well. Ensure the cells you are using are viable and healthy. Try not to let your cells sit for longer than 5 minutes prior to transfection; cell clumping at the time of transfection may reduce transfection efficiency.
2. Briefly pipette mRNA-LNP solution up and down to resuspend. Add 20 uL of the mRNA-LNP mix dropwise directly to your 1 mL culture. Gently tilt plate back and forth to mix (not necessary if you are using cells which will be immediately placed back into a shaker). Place your transfected cells back into their original culture conditions.
3. Check cell expression by FACS at 24hr intervals after transfection.
*Note: This is a generalized protocol for transfection using mammalian cells. Transfection volume may be scaled down/up proportionately.
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