Clipos™ ANTI-EPCAM/CD3 mRNA Lipid Nanoparticles

Catalog:CDPM09

Unit Size:200 μL

INQUIRY Datasheet

Specifications
Description This product utilizes the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of the Anti-EpCAM/CD3 mRNA to a variety of mammalian cells, including cell cultures which may typically be prone to difficulties in expressing desired proteins. The lipid nanoparticles used are formulated with, SM-102, DSPC, cholesterol, and DMG-PEG2000 at optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. Epithelial cell adhesion molecules (EpCAM) is a glycoprotein that was originally identified as a marker for cancer due to its high expression on rapidly proliferating epithelial cells. CD3 is a surface marker found on all T-lymphocytes. Together these proteins can act as a target for identification. The specificity of the Anti-EpCAM/CD3 protein has rendered it a candid vice for mRNA delivery. mRNA encapsulated in lipid nanoparticle, primarily in PBS (-ca, -mg). Dilute levels of: sodium acetate, ethanol, free mRNA & free lipids.
Lipid composition SM-102/DSPC/cholesterol/DMG-PEG2000
Encoding mRNA xEpcam/CD3 BsAb
In Vitro QC (Cell Types) HEK293S
Technology Applications Bispecific antibody
Storage Store at 4°C
Technical Notes Work with mRNA-LNP on ice. It is important to minimize the time that the product spends at room temperature; after handling product during experiments, return immediately to ice.
Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container; it is important not to spin for too long as this may rupture mRNA-LNP’s. Do not vortex.
mRNA-LNP products should only be handled with certified RNAase-free reagents and consumables; use of filtered pipette tips is highly recommended.
Usage 1. Prior to transfection, in a 12 well culture plate, plate your cells at [0.5E6 cells/mL] in a total of 1 mL per well. Ensure the cells you are using are viable and healthy. Try not to let your cells sit for longer than 5 minutes prior to transfection; cell clumping at the time of transfection may reduce transfection efficiency.
2. Briefly pipette mRNA-LNP solution up and down to resuspend. Add 20 uL of the mRNA-LNP mix dropwise directly to your 1 mL culture. Gently tilt plate back and forth to mix (not necessary if you are using cells which will be immediately placed back into a shaker). Place your transfected cells back into their original culture conditions.
3. Check cell expression by FACS at 24hr intervals after transfection.
*Note: This is a generalized protocol for transfection using mammalian cells. Transfection volume may be scaled down/up proportionately.
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