Clipos™ Cas-9 Dead mRNA Lipid Nanoparticles

Catalog:CDL23-078-L

Unit Size:200 μL

INQUIRY Datasheet

Specifications
Description Cas9 (CRISPR-associated protein 9) is a dual RNA-guided DNA endonuclease associated with the clustered regularly interspaced short palindromic repeat (CRISPR) adaptive immune system originally discovered in Streptococcus pyogenes. The Cas9 protein is widely used as a genome engineering tool together with a small guide RNA (sgRNA) or gRNA to induce site-directed double-strand breaks or insertions in DNA, so-called CRISPR/Cas9 gene editing. CRISPR/Cas9 has been the basis for the development of numerous molecular systems used in research and, increasingly, in medical practice. Point mutations D10A and H840A alter 2 residues important for endonuclease activity of Cas9, resulting in Cas9 inactivation. The mutated Cas9 protein devoid of nucleolytic activity, known as Cas9 dead or dCas9, provide a technology for precise epigenetic DNA modification by delivering functional methylases and acetylases to genomic sites. This product is designed as a tool for the delivery and expression of Cas9-dead mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of Cas9-dead mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery
Lipid composition SM-102/DSPC/Cholesterol/DMG-PEG2000
Encoding mRNA Cas9
In Vitro QC (Cell Types) HEK293S
Technology Applications Protein research
Storage Store at 4°C
Technical Notes Work with mRNA-LNP on ice. It is important to minimize the time that the product spends at room temperature; after handling product during experiments, return immediately to ice.Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container; it is important not to spin for too long as this may rupture mRNA-LNP’s. Do not vortex. mRNA-LNP products should only be handled with certified RNAase-free reagents and consumables; use of filtered pipette tips is highly recommended
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