Clipos™ DiR labeled Phosphatidylcholine (PC) Liposomes


Catalog:CDL6001F-DR

Unit Size:1 mL, 5 mL

INQUIRY Datasheet

Specifications
Description The fluorescent control liposomes contain the lipophilic Near IR dye DiR incorporated in the bilayers. It is used for tracking the cellular uptake of the liposomes in vitro and in vivo by common methods like confocal, FACS, NIR imaging etc. The near IR fluorescent, lipophilic carbocyanine DiOC18(7) ("DiR") is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. The sulfonate groups incorporated into this DiI analog improves water solubility. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments.
Lipid composition DOPC/Cholesterol (54/45 molar ratio)
Liposome Size 100 nm (90-120 nm)
Lipid Molar Concentration 50 mM (50-55 mM)
Fluorophore DiR
Fluorophore Concentration 0.5 mM (0.51 mg/mL)
Excitation 750 nm
Emission 780 nm
Buffer 10% sucrose, 20mM HEPES, pH 7.5 ± 0.2
Storage 2-8 °C
Shelf-life 6 months guaranteed for unopened vials.
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