Clipos™ DiD labeled Phosphatidylcholine (PC) Liposomes


Catalog:CDL6001F-DD

Unit Size:1 mL, 5 mL

INQUIRY Datasheet

Specifications
Description The fluorescent control liposomes contain the lipophilic dye DiD incorporated in the bilayers. It is used for tracking the cellular uptake of the liposomes by common methods like confocal, FACS, etc. The far-red fluorescent, lipophilic carbocyanine DiD is a longer-wavelength DiI analog. It is an oil at room temperature and weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments.
Lipid composition DOPC/Cholesterol (54/45 molar ratio)
Liposome Size 100 nm (90-120 nm)
Lipid Molar Concentration 50 mM (50-55 mM)
Fluorophore DiD
Fluorophore Concentration 0.5 mM (0.48 mg/mL)
Excitation 644 nm
Emission 665 nm
Buffer 10% sucrose, 20mM HEPES, pH 7.5 ± 0.2
Storage 2-8 °C
Shelf-life 6 months guaranteed for unopened vials.
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