Clipos™ Phosphatidylglycerol (PG) Liposomes, Lyophilized


Catalog:CDELPG-1256

Unit Size:10 mg

INQUIRY Datasheet

Specifications
Description Liposomes are extensively used to study the interaction of proteins, peptides and other molecules with the surface of a lipid membrane. One of the parameters that affects this interaction is the charge of the liposomal membrane. Liposomes are always made in aqueous environment and they are sized to the desired size in liquid state using various methods such as high pressure extrusion through nano sized pore track etch membranes. Liposome without water is meaningless. In rare occasions, liposomes are freeze dried and proliposomes are formed in the presence of a lyoprotectant such as trehalose. Using a lyoprotectant is necessary in order to maintain the size of the liposomes after rehydration. Phosphatidylglycerol (PG) is a negatively charged molecule. The amount of the negative zeta potential in the liposomes depends on the molar percentage of the phosphatidylglycerol (PG) in the formulation. Anionic PG-based Liposomes contains products made from 0.5 up to 90 percent DOPG with various amount of negative zeta potentials. The matrix lipid, DOPC, that is used in the formulations does not significantly contribute to the charge of the liposomes. Anionic liposomes are used in blood complement studies and various types of in vitro studies.
Lipid composition DOPG/DOPC (40/60 molar ratio)
Liposome Size 100 nm (after hydration)
Surface Charge Anionic
Appearance White lyophilized powder. Liposomes are formed upon adding water to the freeze dried proliposomes and the appearance of the formulation will be white translucent liquid.
Buffer Lyophilized Phosphate Buffered Saline, 7.4 (after hydration)
Storage Should always be stored in a freezer at at -40 °C or -80 °C. Upon adding water to proliposomes, liposomes are formed.
Shelf-life 6 months.
Technical Notes Liposomes are formed upon hydration of the lyophilized formulation. If the lyophilized liposomes are hydrated with solution containing a water soluble drug then a large percentage of the drug will stay outside of the liposomes and in non-encapsulated form. It is advised to use a micro dialysis cassette or a spin column using the right side beads (depending on the size of your drug) and separate the drug encapsulated liposomes from free drug and perform the drug assay in order to calculate the encapsulation efficiency.
Lyophilized liposomes are mainly recommended to be used with drugs that have a short life in aqueous solution mainly due to hydrolysis. After adding the solution of the drug to lyophilized liposomes, the liposomes should be used immediately.
Lyophilized Liposomes products should be used by scientists who understand liposome formulation and have the proper equipment in order to check the size, separate the non-encapsulated drug and do the proper assays.
Trehalose is used as a lyoprotectant in all freeze dried liposome formulation. The size distribution after hydration of the freeze dried formulation will be around 100 nm.

Related Products
Product Name Catalog Lipid composition Liposome Size Price
Clipos™ Phosphatidylglycerol (PG) Liposomes CDECPG-1655 100% DOPG 100 nm INQUIRY
Clipos™ Phosphatidylglycerol (PG) Liposomes, Lyophilized CDELPG-1264 100% DOPG 100 nm (after hydration) INQUIRY
Clipos™ Anionic Phosphatidylglycerol (PG) Liposomes, PEGylated CDECPGG-1586 DOPC/Cholesterol/DOPG/DOPE-PEG(2000) (60/30/5/5 molar ratio) 100 nm INQUIRY
Clipos™ Phosphatidylglycerol (PG) Liposomes CDECPG-1668 DOPG/DOPC (0.5/99.5 molar ratio) 100 nm INQUIRY
Clipos™ Phosphatidylglycerol (PG) Liposomes, Lyophilized CDELPG-1263 DOPG/DOPC (0.5/99.5 molar ratio) 100 nm (after hydration) INQUIRY
Clipos™ Phosphatidylglycerol (PG) Liposomes CDECPG-1667 DOPG/DOPC (1/99 molar ratio) 100 nm INQUIRY
Clipos™ Phosphatidylglycerol (PG) Liposomes, Lyophilized CDELPG-1262 DOPG/DOPC (1/99 molar ratio) 100 nm (after hydration) INQUIRY
Clipos™ Phosphatidylglycerol (PG) Liposomes CDECPG-1664 DOPG/DOPC (10/90 molar ratio) 100 nm INQUIRY
Products
Fill out the form below
to receive a quote

GET A QUOTE

  • (USA)
  • (Europe)
Cookie Policy | Privacy Policy | Copyright © 2024 CD Bioparticles. All rights reserved.
0
Inquiry Basket
Inquiry