Clipos™ DOTA Encapsulated Liposomes, Non PEGylated

Catalog:CDEDTA-1002

Unit Size:2 mL

Specifications

Description	DOTA encapsulated liposomes are used for remote loading of radioactive divalent ions such as radioactive copper into the liposomes. Cationic ions will not pass through the membrane if there is no pH gradient. Divalent ion will cross the liposome membrane only when there is a flux of proton across of the lipid membrane. Due to the difference in the pH inside and outside of the liposomes, there will be a constant flux of proton from inside of the liposomes to outside of the liposomes. Due to this flux, divalent cations move in the opposite directionfrom outside to inside of liposomes. Liposomes are thermodynamically stable structures and not very permeable to divalent cations after they are formed. If there is no pH gradient divalent cations can not move from outside to inside of the liposomes and they will remain outside and won’t cross the membrane. The driving force for remote loading in proton gradient. The radioactive divalent copper is externally added to the liposomes. The divalent ion passes the membrane using pH gradient method and chelates DOTA.
Lipid composition	DSPC/Cholesterol (60/40 molar ratio)
Liposome Size	100 nm
Lipid Mass Concentration	15 mg/mL
Encapsulated Drug	1,4,7,10-Tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA)
Drug concentration	10 mM
Appearance	DOTA liposome is a white translucent liquid made of nano size unilamellar liposomes. Usually due to the small size of liposomes no settling will occur in the bottom of the vial. The liposomes are packaged in an amber vial.
Buffer	HEPES Buffered Saline, pH 4
Storage	DOTA liposome should always be stored at in the dark at 4 °C, except when brought to room temperature for brief periods prior to animal dosing. DO NOT FREEZE. If the suspension is frozen, the encapsulated drug can be released from the liposomes thus limiting its effectiveness. In addition, the size of the liposomes will also change upon freezing and thawing.
Shelf-life	DOTA liposomes are made on daily basis. The batch that is shipped is manufactured on the same day. It is advised to use the products within 4 months of the manufacturing date.