Clipos™ Doxorubicin Encapsulated Liposomes, Biotin lipids, PEGylated

Catalog:CDEIMD-1008

Unit Size:2 mL, 5 mL

INQUIRY Datasheet

Specifications
Description Biotinylated liposomes can be conjugated noncovalently with (strept)avidin through either direct interaction with the protein/antibody conjugated to (strept)avidin or by coupling with other biotinylated proteins using (strept)avidin as a bridging molecule.Both avidin and streptavidin form strong noncovalent bond with biotin. The high resistance to breakdown makes them very useful in bioconjugate chemistry. However, streptavidin has replaced avidin in most bioconjugation applications due to its enhance properties. NeutrAvidin (ThermoFisher) is a modified avidin without negative properties. It performs much better than original avidin and sometimes streptavidin.
In order to exploit the high-affinity interaction of biotin with strept(avidin), a two-step “sandwich” protocol (Method A) has been developed for the preparation of targeted immunoliposomes. In this methodology, (strept)avidin is first attached to biotinylated liposomes, then a biotin-modified protein/antibody is introduced into the biotinylated strept(avidin)-labeled liposomes. This noncovalent approach is rapid, extremely versatile and applicable to numerous targeting ligands of interest with respect to in vitro and in vivo applications. Alternatively, instead of forming a strept(avidin) bridge, strept(avidin) molecule can also be covalently conjugated to antibody or ligand (Method B) and non-covalently bound to liposomes containing biotin on surface in order to form immunoliposomes.
Lipid composition HSPC/Cholesterol/DSPE-PEG(2000)/DSPE-PEG(2000)-Biotin (57/38/4/1 molar ratio)
Liposome Size 100 nm
Lipid Mass Concentration 15.61 mg/mL
Lipid Molar Concentration 21.58 mM
Functional Group Biotin
Encapsulated Drug Doxorubicin
Drug concentration 2 mg/mL (3.45 mM)
Appearance The product is a red translucent liquid made of nano size unilamellar liposomes. Usually due to the small size of liposomes no settling will occur in the bottom of the vial. The liposomes are packaged in an amber vial.
Inside Buffer Ammonium Sulfate, pH 7.4
Outside Buffer Phosphate Buffered Saline, pH 7.4
Storage Should always be stored at in the dark at 4 °C, except when brought to room temperature for brief periods prior to animal dosing. DO NOT FREEZE. If the suspension is frozen, the encapsulated drug can be released from the liposomes thus limiting its effectiveness. In addition, the size of the liposomes will also change upon freezing and thawing.
Shelf-life 4 months.
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