Clipos™ Control of Anionic Clodronate Encapsulated Liposomes, DiR Labeled
Specifications
Description
|
The fluorescent control liposomes contain the near IR fluorescent dye, lipophilic carbocyanine DiOC18(7) ("DiR") incorporated in the bilayers. It has comparable physical and chemical attributes as for the Clodronate Encapsulated Liposomes (Anionic) except no clodronate encapsulated inside. It is used for tracking the cellular uptake of the liposomes by common methods like confocal, FACS, NIR imaging etc. The near IR fluorescent, lipophilic carbocyanine DiOC18(7) ("DiR") is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. The sulfonate groups incorporated into this DiI analog improves water solubility. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. |
Lipid composition
|
PC/PG/Cholesterol |
Fluorophore
|
DiR |
Fluorophore Concentration
|
0.15 mM (0.15 mg/mL) |
Excitation
|
750 nm |
Emission
|
780 nm |
Storage
|
2-8 °C |
Shelf-life
|
6 months for unopened vials |
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