Neutralized Type I Bovine Atelocollagen
Specifications
Description
|
The product is ready-to-use bovine atelocollagen solution that forms a 3D hydrogel by simply warming to 37°C. This product is pH neutral and isotonic formulated with DMEM/F12 medium and ready for immediate cell addition and 3D hydrogels. The product is designed to improve gel consistency by pre-formulation of the medium and adjustment of the product to a neutral pH. This product avoids the inconsistencies in the preparation of the gel that can arise through variables of reagent addition, pH adjustment and handling conditions. This product is sterile and is ideal for providing a firm gel and can also be used in the preparation of a thin layer for culturing cells. This product is for R&D use only and is not intended for human or other uses. |
Storage
|
2-10°C |
Shelf-life
|
Minimum of 6 months from date of receipt. |
Form
|
Solution |
Purity
|
>99% (Silver Staining) |
pH
|
6.9-7.4 |
Cell Attachment Assay
|
Pass |
Concentration
|
~5 mg/mL (Biuret) |
Electrophoretic Pattern
|
Characteristic (Coomassie Blue) |
Endotoxin
|
<1.0 EU/mL (LAL) |
Extraction Method
|
Enzyme-atelocollagen |
Fibrillogenesis
|
>0.5 absorbance units |
Gel Formation Tube Test
|
<40 min |
Hydrogel Young's Modulus E
|
Characteristic |
Kinetic Gel Test
|
<40 min |
L-Glutamine Source
|
Mixture of L-Glutamine and Dipeptide (L-alanine-L-glutamine) |
Medium Supplement
|
DMEM/F-12 |
Osmolality
|
300-360 mOsmo H2O/kg |
Source
|
Bovine Hide |
Sterility
|
No growth (USP) |
Sterilization Method
|
Filtration |
More Info & Documentation
User Manual_Neutralized Type I Bovine Atelocollagen Solution |
Download |
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