MA Collagen with LAP Kit

Methacrylated Collagen with LAP Kit

Catalog:CDAD41

Unit Size:1 Kit

INQUIRY Datasheet

Specifications
Description A methacrylated Type I collagen kit.These 3D collagen gels can be prepared at various concentrations and photocrosslinked (405nm) to provide various gel stiffness. Each kit contains methacrylated collagen, LAP photoinitiator, acetic acid and a neutralization solution. The methacrylated Type I collagen is produced from telo-peptide intact bovine collagen where the collagen has been modified by reacting the free amines, primarily the ε-amines groups of the lysine residues as well as the a-amines groups on the N-termini. > 20% of the total lysine residues of the collagen molecule have been methacrylated. The collagen is extracted from bovine hide and contains a high monomer content. The collagen starting material was isolated from a closed herd and purified using controlled manufacturing processes. A bottle of 20 mM acetic acid solution is provided to solubilize the lyophilized methacrylated collagen at concentrations ranging from 3 to 8 mg/ml. The neutralization solution consists of an alkaline 10X phosphate buffered saline (PBS) solution which provides physiological salts and pH in the final mixture. The photoinitiator consists of LAP to be formulated in 1X cell culture media or PBS which allows blue light photocrosslinking of the collagen at 405 nm. To sterile filter, resuspend and filter through a 0.2 micron button filter. This product is for R&D use only and is not intended for human or other uses.
Component • Methacrylated Collagen, 100 mg
• 20 mM Acetic Acid, 50 mL
• Neutralization Solution, 10 mL
• LAP Photoinitiator, 100 mg
Storage 2-10°C
Shelf-life Minimum of 6 months from date of receipt. 2 month after reconstitution.
Form Lyophilized Powder
Purity >99% (Silver Staining)
Degree of Methacrylation >20%
Electrophoretic Pattern Characteristic (Coomassie Blue)
Endotoxin <10.0 EU/mL (LAL)
Extraction Method Acid-Telocollagen
Gel Formation Tube Test <40 min
Hydrogel Young's Modulus E Characteristic
Kinetic Gel Test <40 min
Source Bovine Hide
Sterility No growth (USP)
Sterilization Method Filtration
More Info & Documentation
User Manual_Methacrylated Collagen with LAP Kit Download
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