10x CB Buffer (pH 7.2)

10x CB Buffer (pH 7.2)

Catalog:CDAS025

Unit Size:600 µL

INQUIRY Datasheet

Specifications
Description 10x CB-Buffer (pH 7.2) is a component of the Hydrogel system. It can be used in the Hydrogel system, where it provides a cell-compatible environment at pH 7.2, which is optimal for maintenance of viable cells. Hydrogels are generated by mixing the liquid components of the system at physiological conditions in the presence of cells, where thiol groups form stable thioether bonds with maleimide groups, which results in the formation of a gel with embedded cells. Prior to the crosslinking step, peptides (e.g. RGD Peptide) can be covalently attached to a portion of the maleimide groups of Maleimide-PVA or Maleimide-Dextran. Sufficient for 3 mL Hydroge. Intended for research use only. Not for use in human therapeutic or diagnostic applications.
Storage -20°C to -80°C
Form Solution
Composition 10 g/L glucose; 0.5 mol/L 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES); 0.05 mol/L KCl; 1.1 mol/L NaCl; 0.2 mol/L NaH2PO4; 0.2 g/L phenol red; pH-adjusted with NaOH.
Sterilization Method Filtration
Applications Note 10x CB-Buffer (pH 7.2) with other components of the 3D Cell Culture Hydrogel System to establish 3-D cell cultures in biomimetic hydrogels.
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