Soft Stiffness Hydrogel Kit

Soft Stiffness Hydrogel Kit

Catalog:CDAS022

Unit Size:1 Kit

INQUIRY Datasheet

Specifications
Description The Soft Stiffness Hydrogel Kit contains reagents for the preparation of hydrogels with a soft stiffness (400 Pa; shear modulus) and a RGD Peptide concentration of 0.4 mmol/L. The gel composition allows three-dimensional spreading and migration of most cells. The gel is formed by the crosslinking of modified dextran carrying the cell adhesion motif RGD (RGD-Dextran) and 3CG-Link. When the two reagents are combined, thiol groups on 3CG-Link form stable thioether bonds with SH-reactive groups on RGD-Dextran resulting in gel formation within 20 min. The formation of hydrogel is performed at physiological pH for optimal cell compatibility. 3CG-Link is composed of polyethylene glycol fused to a matrix metalloprotease (MMP)-cleavable peptide. The MMP-cleavable peptide is designed for a broad range of MMP cleavage including MMP1, MMP3, MMP7 and MMP9*. It allows cells to spread and migrate within the gel if they express the indicated MMPs. The hydrogels can be dissolved by the addition of dextranase (Dextranase Cat. No. CDAS016), which allows recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or further cultivation. Allows formation of 2.4 ml Hydrogel. Intended for research use only. Not for use in human therapeutic or diagnostic applications.
Component • RGD-Dextran, lyophilized, 2 x 900 µL after reconstitution
• 3CG-Link, lyophilized, 200 µL after reconstitution
• Reconstitution Buffer, 2 x 900 µL
• Water, 1000 µL
Reconstitution RGD-Dextran:
Briefly centrifuge lyophilized components to make sure that the entire material is at the bottom of the centrifuge tube.
Add 853 µL Reconstitution Buffer per centrifuge tube. This will result in 900 µL RGD-Dextran per tube. Close tube and wait 5 minutes until all material is dissolved. Vortex briefly.
Let sit reconstituted RGD-Dextran for 1 hr at room temperature.
RGD-Dextran is now ready for use.

3CG-Link:
Briefly centrifuge lyophilized components to make sure that the entire material is at the bottom of the centrifuge tube.
Add 188 µL Water. This will result in a 200 µL 3CG-Link solution. Close centrifuge tube and vortex briefly.
Wait 5 minutes or until all material is dissolved.
Briefly vortex and centrifuge again.
3CG-Link is now ready for use.
Form Lyophilized Powder/Solution
Sterilization Method Filtration
Applications Note 3-dimensional cultivation of various cell types, spheroids, tissue samples and similar requiring a cell-adhesive and cell-degradable matrix for cell growth and differentiation.
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