CD Bioparticles’ products with deep understanding of the strategies and the applications of various of delivery cargos with precise designs and advanced technical platforms can help you to solve:
The challenges you might meet:
- Lack of tissue-specific or cell-specific targeting
- Hard to incorporate active biomolecules, such as peptide, antibody, protein, nucleic acid, and polysaccharide on the surface of the liposomes
- Limited conjugation options
- Antibody denatured during the conjugation
- Tedious chemical synthesis, formulation, and purifications
- Not all chemistries have the same yield and efficiency of conjugation and often reproducing biocompatible batches can be a challenge
Key Features
- PDP Liposomes
- Sulfhydryl Reactive
- The pyridyldithiopropionate (PDP) group contains disulfide, which can react with sulfhydryl or thiolated proteins/antibodies. Therefore, PDP-functionalized liposomes can be used in two ways: A) maleimide-modified antibody to a PDP-modified liposome; B) thiol-modified antibody to a PDP-modified liposome.
- DBCO Liposomes
- The conjugation chemistry is based on the copper-free click reaction of the dibenzocyclooctyne (DBCO) reagent with an azide linker to form a stable triazole. Most efficient and biocompatible conjugation reaction
- Azide Liposomes
- The conjugation chemistry is based on the copper-free click reaction of the dibenzocyclooctyne (DBCO) reagent with an azide linker to form a stable triazole. Most efficient and biocompatible conjugation reaction
- Amine Liposomes
- Available for the conjugation with C-terminal of the biomolecules through EDC reaction, or suitable for the reaction with vinyl molecules via Michael addition.
- Folate Liposomes
- Folate binding protein (FBP) is an endogenous protein, which shows a very high affinity for folate
- Cyanur Liposomes
- Cyanuric chloride-activated DSPE can be conjugated with amine-containing biomolecules or nanoparticles via a nucleophilic substitution reaction under basic conditions
- Succinyl Liposomes
- 4-carbon spacer with carboxylic acid terminals, useful for EDC/NHS conjugation with the amino groups on the biomolecules.
- Glutaryl Liposomes
- 6-carbon spacer with carboxylic acid terminals, useful for EDC/NHS conjugation with the amino groups on the biomolecules.
- Dodecanyl Liposomes:
- 12-carbon spacer with carboxylic acid terminals, useful for EDC/NHS conjugation with the amino groups on the biomolecules.
- Ni Reactive Liposomes:
- to improve and control the association of diverse histidine-tagged peptides to liposomes containing metal-chelating lipids. His-tagged proteins or peptides. the liposomes with Ni-NTA headgroups are combined with the His residues, typically at the N- or C-terminus of proteins, the proteins reversibly anchor to the liposomes.
- Biotinylated Liposomes:
- Biotinylated liposomes can be conjugated noncovalently with (strept)avidin through either direct interaction with the protein/antibody conjugated to (strept)avidin or by coupling with other biotinylated proteins using (strept)avidin as a bridging molecule. The high resistance to breakdown makes them very useful in bioconjugate chemistry
- Carboxylic Acid Liposomes
- Available for the conjugation with N-terminal of the biomolecules through EDC reaction
Key benefits:
- Cell-membrane mimic liposomes
- Multi-functional-group useful for further conjugation and crosslinking, good for further conjugation, labeling, targeting
- Efficient conjugation reaction in the ambient condition to prevent the protein denatured
- Various of conjugators on the liposome surfaces not requiring specific tags on your proteins
Application candidates:
- Bioconjugation with active biomolecules, such as peptide, protein, antibody and nucleic acid to improve the targeted delivery
- Active biomolecules conjugated with lipid molecules assisting the biomolecules assembly into nanoparticles and providing a physical protection
