Retroviral Vectors

CD Bioparticles is a provider of comprehensive solutions for retroviral vectors products and packaging services. These services are primarily designed for researchers to enable them to effectively conduct gene transfer experiments. We pride ourselves on our utilization of pseudotyped retroviral vectors that are derived from two of the most popular viral strains in the scientific community, Moloney Murine Leukemia Virus (MMLV), and Murine Stem Cell Virus (MSCV). The scientists and experts of our team have many years of experience in the field of gene transfer and have developed a comprehensive range of retroviral vectors products and packaging services that cater to your specific experimental requirements. Our team guarantees rigorous testing and impeccable manufacturing processes to ensure the final products match the highest standards.

Introduction to Retroviral Vectors

The retroviridae family (including gamma-retrovirus and lentivirus) is the most common viral vectors. Retroviruses are positive RNA viruses. Their name comes from their ability to reverse transcribe their genome and insert it into the host cell^[1]. Here we mentioned is gamma-retrovirus. The most commonly used method for production of retrovirus in laboratories is transient transfection of 293T cells with a lentivirus vector plasmid, packaging genome plasmid(s), and an envelope expression plasmid. High titer stocks are obtained by concentration of vector supernatants by ultracentrifugation.

The Specific Features of Retroviral Vectors Include:

• The DNA of the retrovirus can be randomly integrated into the host chromosome
• Retroviral vectors enable stable long-term transgene expression.
• Retroviral vectors can only infect dividing cells, but lentiviral vectors, a more complex genus of the retroviridae family, can infect non-dividing cells as well^[2].

The Applications of Retroviral Vectors Include:

Figure 1. Application of Gamma-retrovirus.

• Basic research: Now the retrovirus system has been widely used in gene over-expression in various cell lines, RNA interference, gene research and live animal experiments (Figure 1).
• Construction of stable cell lines: Retroviruses can be used to create stable cell lines to express target proteins. Because they have the ability to integrate into the host genome in a stable fashion.
• Gene therapy: Retroviruses have been used in a number of FDA-approved clinical trials. Retroviruses are suitable for dividing cells, such as stem cell-related research, neural stem cells and hematopoietic stem cells.

Our Retroviral Vectors Featured Services:

Figure 2. A schematic overview of the wt-RV and Pseudotyped RV.

CD Bioparticles have developed a series of proprietary technologies and reagents to improve the level of Retrovirus packaging technology in terms of virus purity, titer, activity and consistency.

We Can Provide:

• VSV-G pseudotyped MMLV
• VSV-G pseudotyped MSCV

Our vectors contain a promoter in the 5' LTR to drive high expression or a self-inactivating 3' LTR to drive high expression of your transgene from the internal CMV promoter.

• Testing in vitro and in vivo: Infection rate, gene expression assessment, immunogenicity assessment, off-target evaluation, toxicological evaluation, etc.

Quotations and Ordering

References

1. Krebs AS, Mendonca LM; et al. Structural Analysis of Retrovirus Assembly and Maturation. Viruses. 2021, 14(1).
2. Arabi F, Mansouri V; et al. Gene therapy clinical trials, where do we go? An overview. Biomed Pharmacother. 2022, 153:113324.