CD Bioparticles is a provider of comprehensive solutions for retroviral vectors products and packaging services. These services are primarily designed for researchers to enable them to effectively conduct gene transfer experiments. We pride ourselves on our utilization of pseudotyped retroviral vectors that are derived from two of the most popular viral strains in the scientific community, Moloney Murine Leukemia Virus (MMLV), and Murine Stem Cell Virus (MSCV). The scientists and experts of our team have many years of experience in the field of gene transfer and have developed a comprehensive range of retroviral vectors products and packaging services that cater to your specific experimental requirements. Our team guarantees rigorous testing and impeccable manufacturing processes to ensure the final products match the highest standards.
The retroviridae family (including gamma-retrovirus and lentivirus) is the most common viral vectors. Retroviruses are positive RNA viruses. Their name comes from their ability to reverse transcribe their genome and insert it into the host cell. Here we mentioned is gamma-retrovirus. The most commonly used method for production of retrovirus in laboratories is transient transfection of 293T cells with a lentivirus vector plasmid, packaging genome plasmid(s), and an envelope expression plasmid. High titer stocks are obtained by concentration of vector supernatants by ultracentrifugation.
Figure 1. Application of Gamma-retrovirus.
Figure 2. A schematic overview of the wt-RV and Pseudotyped RV.
CD Bioparticles have developed a series of proprietary technologies and reagents to improve the level of Retrovirus packaging technology in terms of virus purity, titer, activity and consistency.
Our vectors contain a promoter in the 5' LTR to drive high expression or a self-inactivating 3' LTR to drive high expression of your transgene from the internal CMV promoter.