Silencing

Gene silencing is the practice of having certain genes whose expression is not expressed or can be reduced by others. Gene silencing was first observed in transgenic plants, then in nematodes, fungi, hydroids, fruit flies and mammals. After entering the nucleus, foreign genes will be affected by a variety of factors, which can be divided into three types according to their different action mechanisms and levels: the position effect, transcriptional gene silencing (TGS) at the transcriptional level and transcriptional gene silencing (PTGS) at the post-transcriptional level. Gene silencing is an important pathway for gene expression regulation and a self-protective mechanism of organisms at the level of gene regulation. It is common in foreign DNA invasion, viral infection, DNA transposition and rearrangement. An in-depth study of gene silencing can help one to further reveal the nature of genetic expression regulation in organisms and overcome the phenomenon of gene silencing so that foreign genes can be expressed more efficiently according to human needs. Gene silencing is of extreme theoretical and practical importance as it can effectively suppress the expression of harmful genes in gene therapy for the purpose of disease treatment.

CD Bioparticles' services with customized delivery strategies, precise designs and modifications of drugs or drug-contained cargos, and advanced technical platforms can help you to solve:

The challenges you might meet:

• siRNA, which is involved in the circulation of body fluids, is easily degraded by nucleases in the blood and tissues and loses its function
• Exogenous siRNA is easily recognized and captured by the organism's own immune system
• High molecular weight and electronegativity prevent siRNA from being directly taken up by body cells
• The siRNA delivery vector has high toxicity
• The delivery efficiency of siRNA in targeted tissues and cells is low
• The virus vector has carcinogenic effect and strong immune rejection reaction
• The endosome escape efficiency of siRNA is low in non-viral delivery vectors
• Nonspecific biological distribution of exposed nucleic acids

Silencing Products Key features:

• Magnetofection in Gene Silencing Magnetic beads involved in siRNA transport
• Lipofection in Gene Silencing  Transporter lipids of siRNA

Silencing Products Key benefits:

• Low immune rejection
• High selectivity and little toxic side effects
• High transfection efficiency and good biocompatibility
• No resistance to the drug
• siRNA is not easily degraded in body fluids and can be transported to target tissues and cells
• Large-scale production can be achieved
• Suitable for in vitro and in vivo experiments
• Ready-to-use

Silencing Products Application candidates:

• Treatment of diseases associated with gene expression disorders
• Treatment of HIV infection related diseases
• To reveal the regulation mechanism of cell's own anti-inflammatory response
• Genetic breeding, resistance of crops to fungal diseases
• To treat malignancies such as nasopharyngeal, lung, ovarian, laryngeal and breast cancers
• Research in functional genomics, cell signal transduction, protein interaction and other fields
• Drug discovery and high-throughput screening