Influence of Lipid Composition of Cationic Liposomes 2X3-DOPE on mRNA Delivery into Eukaryotic Cells

Vysochinskaya, V; Shishlyannikov, S; Zabrodskaya, Y; Shmendel, E; Klotchenko, S; Dobrovolskaya, O; Gavrilova, N; Makarova, D; Plotnikova, M; Elpaeva, E; Gorshkov, A; Moshkoff, D; Maslov, M; Vasin, A

Abstract

The design of cationic liposomes for efficient mRNA delivery can significantly improve mRNA-based therapies. Lipoplexes based on polycationic lipid 1,26-bis(cholest-5-en-3 beta-yloxycarbonylamino)-7,11,16,20-tetraazahexacosane tetrahydrochloride (2X3) and helper lipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) were formulated in different molar ratios (1:1, 1:2, 1:3) to efficiently deliver model mRNAs to BHK-21 and A549. The objective of this study was to examine the effect of 2X3-DOPE composition as well as lipid-to-mRNA ratio (amino-to-phosphate group ratio, N/P) on mRNA transfection. We found that lipoplex-mediated transfection efficiency depends on both liposome composition and the N/P ratio. Lipoplexes with an N/P ratio of 10/1 showed nanometric hydrodynamic size, positive zeta potential, maximum loading, and transfection efficiency. Liposomes 2X3-DOPE (1:3) provided the superior delivery of both mRNA coding firefly luciferase and mRNA-eGFP into BHK-21 cells and A549 cells, compared with commercial Lipofectamine MessengerMax.

Keywords: cationic lipids; mRNA; transfection; liposomes; non-viral delivery systems

Related products/services

Cationic Lipids (Transfection)

For more product/services information, please contact us at:

Tel: (USA)

Tel: (Europe)

Fax:

Email: