Reactive Liposomes

CD Bioparticles’ products with deep understanding of the strategies and the applications of various of delivery cargos with precise designs and advanced technical platforms can help you to solve:

The challenges you might meet:

• Lack of tissue-specific or cell-specific targeting
• Hard to incorporate active biomolecules, such as peptide, antibody, protein, nucleic acid, and polysaccharide on the surface of the liposomes
• Limited conjugation options
• Antibody denatured during the conjugation
• Tedious chemical synthesis, formulation, and purifications
• Not all chemistries have the same yield and efficiency of conjugation and often reproducing biocompatible batches can be a challenge

Reactive Liposomes Key Features

• PDP Liposomes
  • Sulfhydryl Reactive
  • The pyridyldithiopropionate (PDP) group contains disulfide, which can react with sulfhydryl or thiolated proteins/antibodies. Therefore, PDP-functionalized liposomes can be used in two ways: A) maleimide-modified antibody to a PDP-modified liposome; B) thiol-modified antibody to a PDP-modified liposome.
• DBCO Liposomes
  • The conjugation chemistry is based on the copper-free click reaction of the dibenzocyclooctyne (DBCO) reagent with an azide linker to form a stable triazole. Most efficient and biocompatible conjugation reaction
• Azide Liposomes
  • The conjugation chemistry is based on the copper-free click reaction of the dibenzocyclooctyne (DBCO) reagent with an azide linker to form a stable triazole. Most efficient and biocompatible conjugation reaction
• Amine Liposomes
  • Available for the conjugation with C-terminal of the biomolecules through EDC reaction, or suitable for the reaction with vinyl molecules via Michael addition.
• Folate Liposomes
  • Folate binding protein (FBP) is an endogenous protein, which shows a very high affinity for folate
• Cyanur Liposomes
  • Cyanuric chloride-activated DSPE can be conjugated with amine-containing biomolecules or nanoparticles via a nucleophilic substitution reaction under basic conditions
• Succinyl Liposomes
  • 4-carbon spacer with carboxylic acid terminals, useful for EDC/NHS conjugation with the amino groups on the biomolecules.

• Glutaryl Liposomes
  • 6-carbon spacer with carboxylic acid terminals, useful for EDC/NHS conjugation with the amino groups on the biomolecules.
• Dodecanyl Liposomes:
  • 12-carbon spacer with carboxylic acid terminals, useful for EDC/NHS conjugation with the amino groups on the biomolecules.
• Ni Reactive Liposomes:
  • to improve and control the association of diverse histidine-tagged peptides to liposomes containing metal-chelating lipids. His-tagged proteins or peptides. the liposomes with Ni-NTA headgroups are combined with the His residues, typically at the N- or C-terminus of proteins, the proteins reversibly anchor to the liposomes.
• Biotinylated Liposomes:
  • Biotinylated liposomes can be conjugated noncovalently with (strept)avidin through either direct interaction with the protein/antibody conjugated to (strept)avidin or by coupling with other biotinylated proteins using (strept)avidin as a bridging molecule. The high resistance to breakdown makes them very useful in bioconjugate chemistry
• Carboxylic Acid Liposomes
  • Available for the conjugation with N-terminal of the biomolecules through EDC reaction

Reactive Liposomes Key benefits:

• Cell-membrane mimic liposomes
• Multi-functional-group useful for further conjugation and crosslinking, good for further conjugation, labeling, targeting
• Efficient conjugation reaction in the ambient condition to prevent the protein denatured
• Various of conjugators on the liposome surfaces not requiring specific tags on your proteins

Reactive Liposomes Application candidates:

• Bioconjugation with active biomolecules, such as peptide, protein, antibody and nucleic acid to improve the targeted delivery
• Active biomolecules conjugated with lipid molecules assisting the biomolecules assembly into nanoparticles and providing a physical protection