Phospholipids are generally white or light yellow powders or lumps at room temperature. They are very soluble in chloroform and can also be dissolved in ether, n-hexane or ethanol, and are almost insoluble in water or acetone. Disperse the phospholipid molecules into the aqueous solution by hydration treatment after high dispersion, or changing the solvent. When the concentration reaches the critical micelle concentration (CMC), self-assembly occurs through the hydrophobic interaction to form a bilayer structured liposome bubble. Commonly used preparation methods of liposomes in the laboratory include thin film method, reverse evaporation method, double emulsion method, centrifugal method, injection method, calcium fusion method, ammonium sulfate gradient method, etc. In order to ensure the final smooth transition to…