With the application of nanotechnology in the field of medicine, lipid- or polymer-based nanocarriers are becoming the mainstream for delivering small-molecule drugs and large molecules, which has increased the effectiveness of drugs and simplified their administration. Nano-scale carriers not only The advantages of nanomaterials, as well as novel properties and functions, such as the ability to interact with complex cell functions in new ways, can create new biomedical applications. In addition, by designing physicochemical properties or surface modification, nanocarriers have multiple potentials for targeted drug delivery to specific sites. Among them, surface charge is one of the important characteristics of nanoparticles. Positively-charged nanocarriers formed from cationic lipids or polymers are most commonly used in…

Nano-drug delivery systems are constantly exposed to complex physiological environments in vivo. The interaction between nano-materials, proteins and cells, their effects and possible toxicity is the key to evaluate and understand the compatibility and toxicity of nano-materials. Cell-based toxicity evaluation is the main method for the in vitro toxicity evaluation of nanometer drug delivery systems, including cell uptake and processing of nanomaterials, effects on cell signals, interference with membranes, effects on cell electron transfer beams, cytochemical factors and reactive oxygen species (ROS) production, intercellular interaction and intercellular transport, gene regulation, obvious toxic reactions, potential toxicity, and cell necrosis or apoptosis. Toxicology In Vitro Research Method Of Nano Drug Delivery System A variety of…

The encapsulation efficiency is an important parameter of the nanodrugs delivery system and can be calculated by the following formula: Encapsulation efficiency (%) = Weight of the drug in nanoparticles / (Weight of the drug in nanoparticles +Weight of the drug in medium) X100% There are generally two methods for determining the drug encapsulation efficiency in a nanometer drug delivery system: 1) the free drug is separated from the nanocarrier and measured; 2) the free drug and the nanocarrier are not separated and measured directly. The first method requires the encapsulated drug to be stable and it should has no leakage during separation. The separation methods include dialysis, gel column chromatography, high speed centrifugation, centrifugal ultrafiltration and microcolumn centrifugation.…

Zeta potential is a specialized term for kinetic potential in colloidal dispersions, also called electromotive potential (ζ-potential), which is used to characterize the surface potential of nanoparticles. Theoretically, zeta potential is the potential of the sliding surface in the interface bilayer relative to a point in the fluid far from the interface. In other words, zeta potential is the potential difference between the dispersion medium and the fluid-fixed layer attached to the dispersed particles. The potential is caused by the net charge in the area around the sliding plane and also depends on the position of the sliding plane. The higher the Zeta potential (positive or negative), the greater the…

The positioning, timing and constant release of pharmaceutical preparations have always been the focus of pharmaceutics research. Special drug delivery systems are prepared by a variety of physical, chemical, and biological methods to control drug release behavior, release the drug at a fixed site of the organism, release it within a predetermined time, or release at a predetermined rate. Compared with common preparations, the controlled release drug delivery system has the advantages of less administration times, stable blood concentration, less drug irritation, and high bioavailability of the drug. Nanotechnology is used to prepare drug carriers by embedding, encapsulating, adsorbing or chemically binding the nanocarrier system. Specific carrier types include nanoparticles,…

After the material is micro-nanosized, especially in the nano-state, its size is between atoms, molecules and bulk materials, so it is called the fourth state of matter. As nanoparticles have many special properties, people have shown great enthusiasm for the research of nanomaterials. They have synthesized a variety of nano- and nano-composites with advanced functions and outstanding performance, which are widely used in various fields. The fine particle size, large specific surface area, insufficient atomic coordination and high surface energy of the nanoparticles make these surface atoms highly active, extremely unstable, and easy to agglomerate. This agglomerated secondary particles are difficult to exert their nano-effects, making the material less than…